%0 Journal Article
%T Phytochemical Analysis and Contractile Effects of Aqueous and Hydroethanolic Extracts of <i>Anastatica hierochuntica</i> L. (Brassicaceae) on the Isolated Uterus of Mice
%A Belem-Kabr¨¦ Wendkouni Leila Marie Esther
%A Niti¨¦ma Mathieu
%A Odjo Blondine Sa£¿dath
%A Maman Noura Oumarou
%A Yaro Boubacar
%A Kabor¨¦ Boukar¨¦
%A Somda Ga£¿tan Donz¨¦o
%A Traor¨¦ Tata Kadiatou
%A Koala Moumouni
%A Ilboudo Sylvain
%A Ou¨¦draogo Moussa
%A Kabr¨¦ Elie
%A Ou¨¦draogo Noufou
%J Pharmacology & Pharmacy
%P 252-270
%@ 2157-9431
%D 2023
%I Scientific Research Publishing
%R 10.4236/pp.2023.148019
%X Background: Anastatica hierochuntica L.
(Brassicaceae) has been claimed to treat various conditions, including
complicated childbirth. The principal objective of this research was to provide
scientific evidence on the contractile effect of the aqueous and hydroethanolic
extracts of the whole plant from A. hierochuntica during labor. Method: Qualitative
and quantitative methods helped identify and quantify the phytoconstituents in
both extracts. The antioxidant activity was studied using four standard methods
(ABTS, DPPH, FRAP, and LPO). Results: The inhibitory power of the
extracts on pro-inflammatory enzymes such as 15-lipoxygenase and phospholipase
A2 was determined. The contractile effects of the extracts were
evaluated using isolated pregnant mice uteri. Steroids, triterpenoids,
flavonoids, tannins, coumarins, and reduced compounds were identified in both
extracts. The hydroethanolic extract exhibited higher content of hydrolyzable
tannins (15.07 ¡À 0.24 mg tannic acid equivalent/g) than the aqueous decoction
(11.8 ¡À 0.69 mg TAE/g). The extracts demonstrated the ability to scavenge DPPH
and ABTS radicals, reduce ferric ions, and inhibit especially lipid peroxidation.
No significant difference was noted in the phospholipase inhibition between the
extracts and betamethasone. The hydroethanolic extract displayed the most
significant anti-lipoxygenase activity with an IC50 value of 55.82 ¡À
1.15 ¦Ìg/mL. The lethal dose (LD50) of extracts was estimated to be
superior to 2000 mg/kg body weight (bw). The ability of the samples to produce
one cm amplitude of contraction at 50% effective concentrations (EC50)
was 5.33 ¡Á 10−8 ¡À 0.32 mg/mL for oxytocin, 1.51 ¡À 0.08 mg/mL for
aqueous extract and 3.57 ¡À 0.61 mg/mL for hydroethanolic extract. Conclusion: These findings could provide evidence for using Anastatica hierochuntica to facilitate
childbirth.
%K <
%K i>
%K Anastatica hierochuntica<
%K /i>
%K Phytoconstituents
%K Antioxidant
%K Anti-Inflammatory
%K Uterine Contractility
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=127117