%0 Journal Article
%T Response Surface Methodology-Based SERS for Determination of Gymnodimine
%A Siyang Chan
%A Yaoyi Wu
%A Yifan Liu
%A Donghang Yin
%A Fei Wang
%J American Journal of Analytical Chemistry
%P 305-325
%@ 2156-8278
%D 2023
%I Scientific Research Publishing
%R 10.4236/ajac.2023.148017
%X Gymnodimine (GYM), a fast-acting marine toxin, is destructive to aquaculture and human health through contaminated shellfish. The current detection methods in GYM have definite drawbacks in operation, such as the demand for delicate instruments and the consumption of time. Therefore, silver colloid was utilized as a surface-enhanced Raman scattering (SERS) desirable substrate for sensitive and rapid detection of GYM in lake and shellfish samples. The theoretical spectrum of GYM is calculated by density functional theory (DFT), and the substrate performance is evaluated by a rhodamine 6 G probe. Under the optimal SERS experimental condition calculated by the response surface methodology, the low limit of detection of 0.105 ¦ÌM with R<sup>2</sup> of 0.9873 and a broad linearity range of 0.1 - 10 ¦ÌM was achieved for GYM detection. In addition, the substrate was satisfyingly applied to detect gymnodimine in the lake and shellfish matrix samples with LOD as low as 0.148 ¦ÌM and 0.170 ¦ÌM, respectively. These results demonstrated a promising SERS platform for detecting marine toxins in seafood for food safety and pharmaceutical research.
%K Surface-Enhanced Raman Scattering
%K Gymnodimine
%K Ag Colloid
%K Box-Behnken Design
%K Response Surface Methodology
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=127298