%0 Journal Article
%T A High-Performance Liquid Chromatography Method for the Simultaneous Determination of Five Index Components in Danhong Injection
%A Yun An
%A Tian Tian
%A Qinglin Wang
%A Xingchu Gong
%A Chenchen Zhang
%J American Journal of Analytical Chemistry
%P 481-492
%@ 2156-8278
%D 2023
%I Scientific Research Publishing
%R 10.4236/ajac.2023.1411028
%X The purpose of this study was to establish a high-performance liquid chromatography (HPLC) method for the simultaneous determination of sodium danshensu, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, and 4-coumaric acid in Danhong injection. The chromatographic method employed was as follows: the column was a Welch Ultimate XB-C18 column (250 mm ¡Á 4.6 mm, 10 ¦Ìm), the mobile phase was a gradient elution of 0.4% formic acid aqueous solution (A) and acetonitrile (B), the detection wavelengths were 280 nm for sodium danshensu, protocatechuic aldehyde, and salvianolic acid B and 326 nm for 4-coumaric acid and rosmarinic acid, the sample volume was 10 ¦ÌL, the flow rate was 1.0 mL/min, and the column temperature was 35¡ãC. This method can realize the separation and determination of sodium danshensu, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, and 4-coumaric acid within 50 minutes. The linear relationships of the five peak areas and their concentrations are good (R<sup>2</sup>&gt; 0.9997). The precision RSD values are all less than 1.0%. The reproducibility RSD values are all less than 1.3%. The stability RSD values are all less than 2.2%. The recovery values ranged from 92.4% to 99.4%. This method is simple, accurate, and reproducible. It can be used for the determination of sodium danshensu, protocatechuic aldehyde, rosmarinic acid, salvianolic acid B, and 4-coumaric acid in Danhong injection.
%K Danhong Injection
%K High Performance Liquid Chromatography
%K Phenolic Acid
%K Flavonoids
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=128769