%0 Journal Article
%T Molecular Identification of <i>Alternaria alternate</i> (Fr.) Keissl. from the Leaf Blight Disease of <i>Centella asiatica</i> L.
%A Nusrat Binte Alam
%A Farhana Rahman
%A Md. Nuhu Alam
%J Natural Science
%P 223-232
%@ 2150-4105
%D 2023
%I Scientific Research Publishing
%R 10.4236/ns.2023.158017
%X <em>Centella asiatica</em> (L.), frequently known as Thankuni, is an important ethnobotanical plant in Bangladesh. This study was conducted to evaluate the morphological characteristics, cultural factors and molecular identification of the causal agent of <em>Alternaria</em> leaf blight disease of <em>C. asiatica</em>. The potato dextrose agar (PDA) medium recorded the maximum mycelial growth (69 mm), followed by the yeast extract agar (YEA) medium, while the honey peptone agar (HPA) medium recorded the lowest growth (27 mm). The optimal pH and temperature for mycelial growth of <em>Alternaria alternata</em> were 6 and 30ˇăC, respectively. Internal transcribed spacer (ITS) region of <em>Alternaria alternata</em> PCR products measured 558 bp and blast search showed 99% sequence similarity with <em>Alternaria alternata</em> species complex. To the best of our knowledge, <em>Alternaria</em> leaf blight disease caused by <em>Alternaria alternata</em> is the first record in Bangladesh.
%K &lt
%K i&gt
%K Alternaria alternata
%K Centella asiatica
%K &lt
%K /i&gt
%K Molecular Identification
%K Mycelial Growth
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=129013