%0 Journal Article
%T Effect of Sijunzi Decoction on the Myonuclear Domain of Rat Soleus in Spleen Qi Deficiency
%A Linlin Zhang
%A Dan Ma
%A Songnan Wang
%A Xudong Liu
%A Lingzhi Wang
%A Dehong Shan
%J Chinese Medicine
%P 276-285
%@ 2151-1926
%D 2023
%I Scientific Research Publishing
%R 10.4236/cm.2023.144015
%X <span><b><span style=\"font-family:&quot;\">Objective</span></b></span><span><span><span style=\"font-family:&quot;\">: To study the mechanism of Sijunzi decoction treating limb weakness in
spleen Qi deficiency (SQD) based on the myonuclear domain (MND) theory. <b>Methods</b>:
40 male Sprague-Dawley rats were randomly divided into the normal group, SQD
model group (model group), SQD+</span></span></span><span><span><span style=\"font-family:&quot;\"> </span></span></span><span><span><span style=\"font-family:&quot;\">still
water group (SW group) and SQD+ Sijunzi decoction group (CM group), 10 rats
each group; Grip-Strength Meter was used to measure limb grip strength;
transmission electron microscope was employed to observe the ultrastructural
changes of the myofibers, Image Pro 6.0 was used to measure the myonuclear
numbers, cross-section area (CSA) and then their ratios (the MND sizes) were
calculated, immunofluorescence assay was chosen to test the expressions of
paired box gene 7 (Pax7) and myogenic differentiation antigen (MyoD). <b>Results</b>:
Compared with those in the normal group, limb grip strength was decreased,
sarcomeres were abnormal, and all the myonuclear numbers, CSA and MND sizes
were reduced, but the Pax7+ cell numbers were increased, significantly, in the
model and SW groups; Compared with those in the model and SW groups, limb grip
strength was increased, sarcomeres were basically normal, the myonuclear number
and CSA were both greater, and the Pax7+ and MyoD+ cell numbers were both
increased, significantly, in the CM group. <b>Conclusion</b>: Sijunzi decoction
might increase the myonuclear number by activating the MSCs to treat limb
weakness in SQD.
%K Sijunzi Decoction
%K Spleen Qi Deficiency
%K Limb Weakness
%K Myonuclear  Domain
%K Muscle Stem Cell
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=129944