%0 Journal Article
%T Phytochemical Profile, Antioxidant, and Anti-Inflammatory Activities, Safety of Use and Spasmolytic Effects of Aqueous Decoction Extract of <i>Diospyros mespiliformis</i> Leaves Hochst. ex A. DC. (Ebenaceae) on the Isolated Duodenum of Rat
%A Mathieu Niti¨¦ma
%A Pegdwend¨¦ Esther Ou¨¦draogo
%A Tata Kadiatou Traor¨¦
%A Oumarou Maman Noura
%A Boukar¨¦ Kabor¨¦
%A Wendkouni Leila Marie Esther B¨¦lem-Kabr¨¦
%A Windingoudi Rimwagna Christian Ou¨¦draogo
%A Moumouni Koala
%A Bruno N¨¦bi¨¦
%A Abdel Magid Ars¨¨ne Ouoba
%A Makumbu Joseph Makaya
%A Sylvain Ilboudo
%A Lazare Belemnaba
%A Noufou Ou¨¦draogo
%A Sylvin Ou¨¦draogo
%J Pharmacology & Pharmacy
%P 513-529
%@ 2157-9431
%D 2023
%I Scientific Research Publishing
%R 10.4236/pp.2023.1412034
%X <i>Diospyros
mespiliformis</i> Hochst. ex A. DC.
(Ebenaceae) is a multi-use plant, including for therapeutic purposes. It is
used in alternative medicine in Burkina Faso to treat conjunctivitis,
menorrhagia, dysentery, and diarrhea. The aim of our study was to evaluate the
chemical profile, antioxidant and anti-inflammatory
activities, safety of use and spasmolytic effects of the aqueous decoction of <i>Diospyros mespiliformis</i> leaves. Phytochemical screening by HPTLC and assay of compounds of interest were carried
out. Four methods were used to assess antioxidant activity. Inhibitory activity
against 15-lipoxygenase and phospholipase A2 was assessed. Acute oral toxicity
of the extract was tested on female mice
(NMRI). Following these tests, the extract contained bioactive compounds of
interest such as flavonoids, tannins, sterols, triterpenes, and saponosides. The total phenolic and flavonoid contents of the aqueous decoctate were
70.59 ¡À 3.20 mg EAT/g and 31.57 ¡À 0.78 mg EQ/g respectively. The extract was
less active than Trolox with inhibitory concentrations of 50% (IC<sub>50</sub>)
for the ABTS, DPPH, FRAP, and LPO tests of 7.53 ¡À 0.08 ¦Ìg/mL, 29.47 ¡À 0.06
¦Ìg/mL, 1128.83 ¡À 4.82 mol EAA/g, and 32.30 ¡À 1.60 ¦Ìg/mL respectively. The
extract has an anti-inflammatory effect with inhibition of phospholipase A2
compared to betamethasone. In addition, the aqueous extract produced an
antispasmodic effect with Emax of 70% and 80% respectively during contractions
induced by BaCl<sub>2</sub> and ACh. Finally, this study provided basic
scientific data and could justify the use of <i>D. mespiliformis</i> leaves in
the treatment of diarrhea.
%K &lt
%K i&gt
%K Diospyros mespiliformis&lt
%K /i&gt
%K Phytoconstituents
%K Antioxidants
%K Acute Oral Toxicity
%K Spasmolytic
%K Leaves
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=130294