%0 Journal Article
%T Long-Term Impact of Acute Retinoic Acid Supplementation at the Young Age on Testicular Architecture of Wistar Albino Rats
%A Mama Sy
%A Racha Kamenda Ibondou
%A Fatoumata Bah
%A Robert Foko
%A Ndiaga Diop
%A Mame V¨¦nus
%A Abdoulaye S¨¦ga
%A Cheikh Diop
%A Mamadou Fall
%A Oumar Faye
%J Advances in Reproductive Sciences
%P 1-13
%@ 2330-0752
%D 2024
%I Scientific Research Publishing
%R 10.4236/arsci.2024.121001
%X Introduction: Inappropriate and excess vitamin supplementation, particularly for
vitamin A, is increasingly recognized as a public health problem in developed
countries. On the other hand, blind supplementation of vitamin A, for children
in developing countries is a subject of controversy in the literature. The
crucial role of vitamin A in the process of spermatogenesis in adult rodents is
well established, but only a few publications are consecrated to the long-term
effect of vitamin A intake at a young age on testicular development and
differentiation. Objectives: Our study aimed to evaluate the long-term
effects of acute supplementation at an early age, in the post-natal period, on
spermatogenesis and testicular trophicity at adult age. Material and
Methods: Young Wistar Albinos rats of 22 days received an acute high dose
of supplementation of vitamin A (retinyl palmitate). The control group, group
1, received only extra virgin olive oil, Group 2 a dose of 7000 IU/kg of
retinyl palmitate, group 3, 14,000
IU/kg, and Group 4 a dose of 28,000
IU/kg. At 10 weeks of age, the testes¡¯ testosterone levels were measured by
ELISA. For histological assessment, sections were stained with Hematoxylin
eosin, and the Johnsen score was used to evaluate spermatogenesis in the
seminiferous tubules. Results: The average testicular weights of rats
were significantly lower in group 4 (p < 0.05), and so was the testosterone
level in the testis compared to the control group (p < 0.01). Most of the
seminiferous tubules were concerned by an arrest of spermatogenesis and the
Johnsen score was decreased with a mean score of 5.96 ¡À 1.60 (p < 0.001) in
that Group. In Group 3, Johnsen¡¯s score was significantly better than the one
obtained with the control. Conclusion: We observed a negative effect in
the long term with a high acute dose of supplementation of retinyl palmitate at
a young age, on testicular development and differentiation. Despite a return
%K Vitamin A
%K Retinyl Palmitate
%K Spermatogenesis
%K Testis-Wistar Albino Rats
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=130166