%0 Journal Article
%T Case Study for Undetermined Mosquito Species by Polymerase Chain Reaction in Western Burkina Faso
%A Koudraogo Bienvenue Yam¨¦ogo
%A Sassan Simplice Kambou
%A Domonbab¨¦l¨¦ Fran£¿ois de Sales Hien
%A Logotio Rachel Angela Traore
%A Franck Adama Yao
%A Rakiswend¨¦ Serge Yerbanga
%J American Journal of Molecular Biology
%P 43-53
%@ 2161-6663
%D 2024
%I Scientific Research Publishing
%R 10.4236/ajmb.2024.142004
%X <b>Introduction: </b>Malaria eradication campaigns all over the world are
largely based on parasite and vector control. Vector identification, whether
morphological or molecular, is an essential component of vector control. This
study analyzed the possible causes of indeterminate polymerase chain reaction
(PCR) results for mosquito species in Western part of Burkina Faso. <b>Methodology:</b> From July 2021 to November 2021, mosquitoes were collected during the period of
high malaria transmission in the village of S¨¦gu¨¦r¨¦, Houet province, Burkina
Faso, and morphologically identified. After DNA extraction, samples were
amplified by sine 200¡Á PCR to identify species of the <i>Anopheles gambiae</i> complex. Indeterminate samples were then selected
for further analysis. The parameters studied were: DNA dilution, the effect of
protocol adjusting, and the type of protocol used. <b>Results: </b>A total of
130 ¡°indeterminate¡± DNAs diluted 1:10 were analyzed. After dilution, the mean
amount was 14.73 ¡À 3.59 ng/¦ÌL and absorbance 1.71 ¡À 0.1. PCR chain reaction
yielded 94.62% (123/130) anopheline species in SINE PCR, 5.38% (7/130) ¡°negative¡±.
A significant difference between SINE PCR before dilution and after dilution
was observed (<i>P</i> &lt; 0.001).
Identification tests carried out using other protocols gave no positive
results. From these results, we note that the adaptation of the protocol
significantly reduced the polymerase amplification results of the species.<b> Conclusion:</b> It is therefore necessary to respect the amplification
protocols. However, the persistence of ¡°indeterminate¡± results suggests that
further studies should be carried out to shed more light on the subject.
%K Malaria
%K Vector
%K DNA
%K PCR
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=131101