%0 Journal Article
%T Phenotypic Detection of Enterobacterales Strains Susceptible of Producing OXA-48 Carbapenemase
%A Abdoulaye Seck
%A Abdou Diop
%A Babacar Ndiaye
%A Assane Dieng
%A Awa Ba
%A Amadou Diop
%A Chantal Mahou Douala-Djemba
%A Thierno Abdoulaye Diallo
%J Advances in Microbiology
%P 115-121
%@ 2165-3410
%D 2024
%I Scientific Research Publishing
%R 10.4236/aim.2024.142009
%X Background: Nowadays, emergence of Carbapenemase-Producing Enterobacterales (CPE) throughout the world has become a public health problem, especially in countries with limited resources. In recent years, CPE of type OXA-48 (Ambler class D) have been identified in Dakar. The aim of this study was to evaluate the phenotypic detection of OXA-48 CPE using a temocillin disc (30 ¦Ìg). Methodology: A retrospective study was carried out at Medical Biology Laboratory of Pasteur Institute in Dakar on Ertapenem-Resistant Enterobacterales (ERE) strains isolated from 2015 to 2017. These strains were then tested with a 30 ¦Ìg temocillin disc. Any strain resistant to temocillin (inhibition diameter < 12 mm) was considered to be OXA-48 CPE. Confirmation of OXA-48 CPE was performed by PCR and sequencing. Results: Forty-one ERE isolated during the study period were tested, of which 34 (82.9%) were OXA-48 based on phenotypic detection using temocillin disc and confirmed by PCR (100%). OXA-48 CPE strains detected were composed of Klebsiella pneumoniae (n = 14; 41.2%), Enterobacter cloacae (n = 8; 23.5%), Escherichia coli (n = 7, 20.5%), Citrobacter freundii (n = 3; 8.8%), Cronobacter sakazakii (n = 1; 3%) and Morganella morganii (n = 1; 3%). Conclusion: Temocillin resistance has a good positive predictive value for detecting OXA-48 CPE by phenotypic method, confirmed by PCR. Temocillin is therefore a good marker for detection of OXA-48 CPE except Hafnia alvei.
%K Ertapenem
%K Temocillin
%K Phenotypic Detection
%K Carbapenemase-Producing Enterobacterales
%K OXA-48
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=131194