%0 Journal Article
%T The Combined Effect of Lumenato and Ceramide in the Protection of Collagen Damage Induced by Neutrophils in Normal Human Dermal Fibroblasts
%A Yulia Solomonov
%A Rachel Levy
%J Advances in Bioscience and Biotechnology
%P 140-159
%@ 2156-8502
%D 2024
%I Scientific Research Publishing
%R 10.4236/abb.2024.152010
%X <b><span style=\"font-family:&quot;\">Introduction</span></b><span style=\"font-family:&quot;\">:
Collagen is the primary structural protein fibroblasts produce in the skin¡¯s
extracellular matrix. Infiltration of neutrophils into the epidermis and dermis
by exposure to UV causes collagen damage and contributes to <span>photoaging. <b>Methods</b>: To study the combined
effect of Lumenato and ceramide </span>in preventing collagen-1 damage induced
by phagocytes, we used co-cultures o<span>f normal
human dermal fibroblasts (fibroblasts) and activate</span>d human neutrophils.
The present study aimed to determine the protective effect of the combination
of Lumenato and ceramide on fibroblast collagen-1 damage induced by
neutrophils. <b>Results:</b> Lumenato (in the range of 6.5</span><span style=\"font-family:&quot;\"> </span><span style=\"font-family:&quot;\">-</span><span style=\"font-family:&quot;\"> </span><span style=\"font-family:&quot;\">208 ¦Ìg/ml) or ceramide (in
the range of 0.1</span><span style=\"font-family:&quot;\"> </span><span style=\"font-family:&quot;\">-</span><span style=\"font-family:&quot;\"> </span><span style=\"font-family:&quot;\">50 ¦ÌM) inhibited the
production of superoxides and MPO by TNF<i>¦Á</i>-stimulated
neutrophils, as well as the production of NO by LPS-stimulated macrophages in a
dose-dependent manner. The combinations of Lumenato and ceramide, in low
concentrations, caused synergis<span>tic prevention
of fibroblasts¡¯ collagen-1 damage induced by TNF<i>¦Á</i>-activate</span>d n<span>eutrophils,
detected by fluorescence immunostaining and WB analys</span>is. MPO activity in
the supernatants of the co-cultures was also synergistically inhibited. Adding
Lumenato or ceramide singly or in combinations in these low concentrations to
the fibroblast cultures did not affect the expression of collagen-1. The combinations
of Lumenato or ceramide in these concentrations al<span>so caused a synergistic inhibition of NO production by activated macro</span>phages. <b>Conclusions</b>: The results suggest that combining low concentrations o<span>f Lumenato and ceramide results in synergistic
protection against fibro</span>blasts¡¯ collagen-1 damage induced by
neutrophils, thus indicating their possible potential for enhanced skin health.
%K Dermal Fibroblasts
%K Neutrophils
%K Collagen-1
%K Lumenato
%K Ceramide
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=131306