%0 Journal Article
%T INF-γ and IL-2 Profile as Therapeutic Markers in Tuberculosis Patients at the Jamot Hospital of Yaounde-Cameroon
%A Alexandra Emmanuelle Membangbi
%A Jacky Njiki-Bikoï
%A Esther Del-Florence Moni Ndedi
%A Donatien Serge Mbaga
%A Elsa Nguiffo Makue
%A André
%A Chris Mikangue Mbongue
%A Koko-Ta Ladiff Sharonne
%A Martha Mesembe
%A Justin Olivier Essindi
%A Aï
%A cha Ngoutane
%A Arnaud Franck Elang
%A Sabine Aimé
%A e Touangnou-Chamda
%A George Ikomey Mondinde
%A Eric Walter Perfura-Yone
%A Marie Joseph Nkodo Mindimi
%A Sara Honorine Riwom Essama
%J Journal of Tuberculosis Research
%P 119-128
%@ 2329-8448
%D 2024
%I Scientific Research Publishing
%R 10.4236/jtr.2024.122008
%X Background: Tuberculosis (TB) is one of the top lethal infectious diseases worldwide. In recent years, interferon-γ (INF-γ) release assays (IGRAs) have been established as routine tests for diagnosing TB infection. However, produced INF-γ assessment cannot permit to distinguish active ATB from latent TB infection (LTBI), especially in TB epidemic areas. In addition to IFN-γ, interleukin-2 (IL-2), secreted by activated T cells, is involved in immune response against Mycobacterium tuberculosis. This could be involved in the follow up of treatment response. The aim of our study was to determine IFN-γ and IL2 cytokines profiles of patients under antituberculosis treatment. Materials and Methods: A six months’ cross-sectional study was conducted at the Jamot Hospital of Yaoundé, from May to August 2021. Sociodemographic and clinical data as well as 5 mL of blood were collected from each participant. INF-γ and IL-2 were determined using indirect Enzyme linked Immuno-Sorbent Assay (ELISA) according to the manufacturer’s recommendations and spectrum exam in combination with radiography and GeneXpert were used as standard. P-values < 0.05 were interpreted as statistically significant. All statistical analysis was performed using SPSS version 22.0 Results: The results showed that men were more infected 14/61 (31.8%) with a high presence in active and resistant TB groups. The mean age was 41.3 ± 13.1 years with a 95% CI = [38.2 - 44.7], the age group with the highest infection rate was ranged between 31 and 40 years. The IL-2 and INF-γ means were respectively 327.6 ± 160.6 pg/mL and 26.6 ± 13.0 pg/mL in ATB patients, 251.1 ± 30.9 pg/mL and 21.4 ± 9.2 pg/mL in patients with resistant tuberculosis, while it was 149.3 ± 93.3 pg/mL and 17.9 ± 9.4 pg/mL in cured patients, 15.1 ± 8.4 pg/mL and 5.3 ± 2.6 pg/mL in participants presumed healthy (p < 0.0001). Significant differences in IFN-γ and IL-2rates were observed between the different groups. Conclusion: Monitoring the serum levels of INF-γ and IL-2 would be useful for the follow-up of anti-tuberculosis patients, particularly in the both cytokines association case.
%K Tuberculosis
%K Antituberculosis Treatment
%K INF-γ
%K
%K IL-2
%K ELISA
%U http://www.scirp.org/journal/PaperInformation.aspx?PaperID=133909