%0 Journal Article
%T 线纹海马<i>Sox9a</i>基因的克隆与表达分析<br>Cloning and Expression Analysis of <i>Sox9a</i> Gene in <i>Hippocampus </i><i>erectus</i>
%A 王苏丹
%A 涂东宇
%A 杨子涵
%A 孙金辉
%A 杨燕菁
%J Open Journal of Fisheries Research
%P 127-135
%@ 2373-1451
%D 2024
%I Hans Publishing
%R 10.12677/ojfr.2024.112016
%X <i>Sox9</i>在脊椎动物性腺分化及精巢发育中行使重要功能。为探究线纹海马<i>s</i><i>ox9a</i>的分子特征及表达模式，本研究采用RACE获得了其cDNA全长1979 bp，包含339 bp 5’ UTR、1488 bp的ORF和152 bp 3’ UTR，共编码496个氨基酸；氨基酸多重比对显示，线纹海马<i>sox</i><i>9</i>具有高度保守的HMG-box结构域；系统进化树分析表明，其与侏儒海马<i>sox9a</i>的亲缘关系最近；RT-qPCR显示，<i>sox9a</i>泛在表达于线纹海马成体的主要组织中，在性腺中呈显著的雄性偏好性高表达于精巢，揭示其可能参与线纹海马精巢的分化及维持。本研究为后续深入研究线纹海马<i>sox</i><i>9</i><i>a</i>在性别发育中的功能奠定了基础。<br />
<i>Sox9</i> plays an important role in gonadal differentiation and testis development in vertebrates. In order to explore the molecular characteristics and expression pattern of <i>sox9a</i> in <i>Hippocampus </i><i>erectus</i>, RACE was used to obtain its full length cDNA of 1979 bp, including 339 bp 5’UTR, 1488 bp ORF and 152 bp 3’UTR, encoding a total of 496 amino acids. Multiple amino acid comparisons showed that the <i>Hippocampus </i><i>erectus</i><i> </i><i>sox9</i> had a highly conserved HMG-box domain. Phylogenetic tree analysis showed that it was most closely related to <i>Hippocampus </i><i>zosterae</i><i> </i><i>sox9a</i>. RT-qPCR showed that<i> sox9a</i> was ubiquitously expressed in the main tissues of <i>Hippocampus </i><i>erectus</i> adults, and showed a significant male preference in the gonads and was highly expressed in the testis, suggesting that <i>sox9a</i> might be involved in the differentiation and maintenance of <i>Hippocampus </i><i>ere</i><i>c</i><i>tus</i> testis. This study laid a foundation for further research on the role of <i>Hippocampus </i><i>erectus</i><i> </i><i>sox9a</i> in sexual development.
%K 线纹海马，<i>Sox9a</i>，性别决定，性别分化<br><i>H</i><i>ippocampus</i><i> </i><i>erectus</i>
%K <i>Sox9a</i>
%K Sex Determination
%K Sex Differentiation
%U http://www.hanspub.org/journal/PaperInformation.aspx?PaperID=90292