%0 Journal Article
%T Expression of VEGF and collagen using a latex biomembrane as bladder replacement in rabbits
%A Domingos
%A Andr¨¦ Lu¨ªs Alonso
%A Garcia
%A S¨¦rgio Britto
%A Bessa Jr.
%A Jos¨¦ de
%A Cassini
%A Marcelo Ferreira
%A Molina
%A Carlos Augusto Fernandes
%A Tucci Junior
%A Silvio
%J International braz j urol
%D 2012
%I Sociedade Brasileira de Urologia
%R 10.1590/S1677-55382012000400014
%X objective: to investigate the vegf expression and collagen deposition using a latex biomembrane as bladder replacement in rabbits. materials and methods: after partial cystectomy, a patch of a non-vulcanized latex biomembrane (2 x 2 cm) was sewn to the bladder of rabbits with 5/0 monofilament polydioxanone sulfate sutures in a watertight manner. groups of 5 animals were killed at 15, 45 and 90 days after surgery and the bladder was removed. sections of 5¦Ìm were cut and stained with picrosirius-red in order to estimate the amount of extracellular matrix in the graft. to confirm the presence of vegf in tissues, protein expression was determined by immunohistochemistry. results: no death, urinary leakage or graft extrusion occurred in any group. all bladders showed a spherical shape. a progressive reduction in the amount of collagen occurred in the graft area and was negatively and linearly correlated with time (p < 0.001). vegf expression was higher in grafted areas when compared to controls at 15 and 45 days after surgery and decreased with time (p < 0.001). conclusion: the latex biomembrane as a matrix for partial bladder replacement in rabbits promotes temporary collagen deposition and stimulates the angiogenic process.
%K latex
%K collagen
%K urinary tract
%K tissue-engineering.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1677-55382012000400014&lng=en&nrm=iso&tlng=en