%0 Journal Article
%T Detecci¨®n y estadificaci¨®n molecular del c¨¢ncer vesical mediante RT-PCR a tiempo real para gelatinasas (MMP-2, MMP-9) y TIMP-2 en sangre perif¨¦rica
%A Angulo
%A J.C.
%A Ferruelo
%A A.
%A Rodr¨ªguez-Barbero
%A J.M.
%A N¨²£¿ez
%A C.
%A Fata
%A F.R. de
%A Gonz¨¢lez
%A J.
%J Actas Urol¨®gicas Espa£¿olas
%D 2011
%I Scientific Electronic Library Online
%R 10.4321/S0210-48062011000300001
%X introduction: molecular staging of bladder cancer based on the detection of mrna of urothelial specific genes in circulating cancer cells has been inconclusive. we analyze whether real-time rt-pcr evaluation of gelatinases (mmp-9, mmp-2) and timp-2 in peripheral blood to diagnose and characterize patients with bladder neoplasm. material and method: total rna is extracted from circulating blood cells in 42 individuals (11 healthy controls, 31 patients with bladder cancer of different stages) and real-time rt-pcr performed using specific primers for mmp-9, mmp-2, timp-2 and ribosomal 18s. the quantification values of mrna are described as relative to 18s mrna (¦Ä¦Äct method) and the results are blindly compared with data obtained from histological diagnosis and clinical staging. results: normalized levels of mmp-9 and mmp-2 mrna are higher in patients with cancer than controls (1.82¡À0.6-fold and 2.7¡À0.6-fold, respectively; p<.05). patients with metastatic disease also have increased mmp-9, mmp-2 and timp-2 mrna levels (9.6¡À0,20-fold, 5.22¡À0.26-fold and 1,97¡À0,22-fold, respectively; p<.05). mmp-9 and mmp-2 are also associated with advanced clinical stage and grade (p<.05). a ratio between variables that increases the ability to segregate patients with ta, t1, t2-4m0 and t2-4m1 tumours is proposed. conclusions: both non-invasive bladder tumor recognition and molecular staging of the disease is possible using real-time rt-pcr-based detection of gelatinases and timp-2 in peripheral blood. the ability to distinguish metastatic disease is higher for mmp-9 but mmp-2 discriminates better levels of tumour invasion. further investigation in this field could yield promising results regarding molecular evaluation of bladder neoplasia.
%K bladder neoplasia
%K molecular staging
%K rt-pcr
%K mmp-2
%K mmp-9
%K timp-2.
%U http://scielo.isciii.es/scielo.php?script=sci_abstract&pid=S0210-48062011000300001&lng=en&nrm=iso&tlng=en