%0 Journal Article
%T Rabbit antivenom efficacy against myotoxic and neurotoxic activities of Bothrops jararacussu venom and bothropstoxin-I
%A Oshima-Franco
%A Y.
%A Leite
%A G. B.
%A Val¨¦rio
%A A. A.
%A Hyslop
%A S.
%A Andriao-Escarso
%A S.
%A Giglio
%A J. R.
%A Prado-Franceschi
%A J.
%A Cruz-H£¿fling
%A M. A.
%A Rodrigues-Simioni
%A L.
%J Journal of Venomous Animals and Toxins
%D 2002
%I Scientific Electronic Library Online
%R 10.1590/S0104-79302002000200004
%X bothrops jararacussu venom and its major toxin bothropstoxin-i (bthtx-i) possess myotoxic and neurotoxic properties. the efficacy of a rabbit antivenom raised against b. jararacussu venom in the neutralization of physiological, biochemical, and morphological changes induced by the venom and its major toxin bthtx-i was studied in mouse isolated phrenic nerve-diaphragm (pnd) and extensor digitorum longus (edl) preparations. the times required for 50% neuromuscular blockade in pnd and edl preparations for venom were 70+11.5 (s.e.m., n=5) min and 58+8 (n=16) (50 m g/ml), and for bthtx-i 31+6 (n=3) min and 30+3 (n=5) min (20 m g/ml), respectively. after 120 min incubation, creatine kinase (ck) concentrations in solution containing the edl preparations were 3464+346 u/l after exposure to venom (50 m g/ml, n=5) and 3422+135 u/l to bthtx-i (20m g/ml, n=4), respectively. rabbit antivenom dose-dependently neutralized venom and toxin-induced neuromuscular blockade in both preparations and effectively prevented venom and toxin-induced ck release from edl. histological analysis showed that rabbit antivenom neutralized morphological damage caused by b. jararacussu venom and bthtx-i in edl preparations. these results indicate that rabbit antivenom effectively neutralized the biological activities of b. jararacussu venom and bthtx-i.
%K bothrops venom
%K myotoxin
%K neutralization
%K neuromuscular junction
%K histological analysis
%K bothrops jararacussu.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0104-79302002000200004&lng=en&nrm=iso&tlng=en