%0 Journal Article
%T Ultra-performance liquid chromatographic method for measurement of voriconazole in human plasma and oral fluid
%A Boeira
%A Paula
%A Antunes
%A Marina V.
%A Andreolla
%A Huander F.
%A Pasqualotto
%A Alessandro C.
%A Linden
%A Rafael
%J Journal of the Brazilian Chemical Society
%D 2012
%I Sociedade Brasileira de Qu¨ªmica, SBQ
%R 10.1590/S0103-50532012000100021
%X a simple, sensitive and selective ultra-performance liquid chromatography method for the determination of voriconazole in plasma and oral fluid was developed and validated. after a liquid-liquid extraction with methyl-tert-butyl ether, the analyte and internal standard were separated on a hypersil gold c18 column (2.1 ¡Á 100 mm, p.d. 1.9 ¦Ìm), eluted with a mobile phase composed of thietylammonium phosphate buffer and acetonitrile (70:30, v/v). total run time was 4 min, total mobile phase consumption of 2.2 ml. detection was performed with a photodiode array detector with quantitation at 256 nm. voriconazole concentrations in oral fluid were on average 57.5% (¡À5.3) of those measured in paired plasma samples.
%K voriconazole
%K uplc-pda
%K oral fluid
%K drug monitoring.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0103-50532012000100021&lng=en&nrm=iso&tlng=en