%0 Journal Article
%T Determination of ursolic acid and ursolic acid lactone in the leaves of Eucalyptus tereticornis by HPLC
%A Maurya
%A Anupam
%A Srivastava
%A Santosh Kumar
%J Journal of the Brazilian Chemical Society
%D 2012
%I Sociedade Brasileira de Qu¨ªmica, SBQ
%R 10.1590/S0103-50532012000300013
%X a simple isocratic hplc method has been developed for the simultaneous quantification of two bioactive triterpenes, ursolic acid and ursolic acid lactone in e. tereticornis leaves. samples were analyzed on rp-18 (4.6 ¡Á 250 mm, 5 ¦Ìm) column with methanol and water acidified to ph 3.5 with tfa (88:12) at 210 nm. the method was validated and applied for the simultaneous quantification of the individual triterpenes in e. tereticornis extract. the calibration curves were linear over a concentration range of 0.05-0.3 mg ml-1 (r = 0.999 and 0.998, respectively). the limits of detection and quantification were 0.190 and 0.644 ¦Ìg for ursolic acid, and 0.176 and 0.587 ¦Ìg for ursolic acid lactone, while the percentage recoveries were 97.32 and 96.23% for ursolic acid and ursolic acid lactone, respectively. this is the first report on the hplc method of ursolic acid lactone with high precision and accuracy.
%K eucalyptus tereticornis
%K hplc
%K triterpenoids
%K ursolic acid
%K ursolic acid lactone.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0103-50532012000300013&lng=en&nrm=iso&tlng=en