%0 Journal Article
%T In vitro effects of 2-methoxyestradiol-bis-sulphamate on cell growth, morphology and cell cycle dynamics in the MCF-7 breast adenocarcinoma cell line
%A Vorster
%A Chris
%A Joubert
%A Annie
%J Biocell
%D 2010
%I Universidad Nacional de Cuyo
%X in the search for new and improved anticancer therapies, researchers have identified several potentially useful compounds. one of these agents is 2-methoxyestradiol-bis-sulphamate (2me-bm), a sulphamoylated derivative of 2-methoxyestradiol. the objective of this study was to evaluate 2me-bm's in vitro efficacy as antiproliferative agent in the mcf-7 breast adenocarcinoma cell line. light- and fluorescent microscopy showed decreased cell density, increased apoptotic characteristics and significant ultrastructural aberrations indicative of autophagic cell death after 24 hours of exposure at a concentration of 0.4¦Ìm. in addition, mitotic indices revealed that 2me-bm induces a g2m block. the latter was confirmed by flow cytometric analyses where increased sub-g1 and g2/m fractions, as well as an increase in cyclin b1 levels were observed. further in vitro research into the mechanism of this potentially useful anticancer compound is thus warranted.
%K cancer
%K 2-methoxyestradiol
%K sulphamoylated estradiol derivative
%K apoptosis
%K autophagy.
%U http://www.scielo.org.ar/scielo.php?script=sci_abstract&pid=S0327-95452010000200003&lng=en&nrm=iso&tlng=en