%0 Journal Article
%T Stabilization of glucose oxidase with cyclodextrin-branched carboxymethylcellulose
%A Matos
%A Madyu
%A Simpson
%A Benjamin K
%A Ram¨ªrez
%A H¨¦ctor L
%A Cao
%A Roberto
%A Torres-Labandeira
%A Juan J
%A Hern¨¢ndez
%A Karel
%J Biotecnolog£¿-a Aplicada
%D 2012
%I Scientific Electronic Library Online
%X we present a methodology for improving some enzymatic characteristics of glucose oxidase (gox) (ec 1.1.3.4). the enzyme was chemically modified with a £¿-cyclodextrin-branched carboxymethylcellulose polymer (cmc-cd), using carbodiimide as coupling agent. the obtained neoglycoenzyme had 0.78 mol of polysaccharide per mol of gox and retained 67% of its initial activity. comparison of some characteristics of the modified and free enzymes showed a higher km for derivatized gox and better thermostability, which increased from 45 ¡ãc to 51 ¡ãc. in addition, derivatization of gox with cmc-cd increased its resistance to inactivation at 45 ¡ãc by 2.2-fold, protected the molecule against inactivation with the anionic surfactant sodium dodecylsulphate to the point that it retained 75% of its activity after an incubation of 3 h, and extended its ph tolerance toward alkaline ph (7.5). covalent glycosidation of glucose oxidase with cd-branched carboxymethylcellulose polymer constitutes therefore an effective strategy for enhancing the stability of this enzyme.
%K carboxymethylcellulose
%K cyclodextrin
%K enzyme stability
%K glucose oxidase.
%U http://scielo.sld.cu/scielo.php?script=sci_abstract&pid=S1027-28522012000100005&lng=en&nrm=iso&tlng=en