%0 Journal Article
%T REG¦Ã is a strong candidate for the regulation of cell cycle, proliferation and the invasion by poorly differentiated thyroid carcinoma cells
%A Zhang
%A M.
%A Gan
%A L.
%A Ren
%A G.S.
%J Brazilian Journal of Medical and Biological Research
%D 2012
%I Associa??o Brasileira de Divulga??o Cient¨ªfica
%R 10.1590/S0100-879X2012007500035
%X reg¦Ã is a proteasome activator that facilitates the degradation of small peptides. abnormally high expression of reg¦Ã has been observed in thyroid carcinomas. the purpose of the present study was to explore the role of reg¦Ã in poorly differentiated thyroid carcinoma (pdtc). for this purpose, small interfering rna (sirna) was introduced to down-regulate the level of reg¦Ã in the pdtc cell line sw579. down-regulation of reg¦Ã at the mrna and protein levels was confirmed by rt-pcr and western blot analyses. facs analysis revealed cell cycle arrest at the g1/s transition, the mtt assay showed inhibition of cell proliferation, and the transwell assay showed restricted cell invasion. furthermore, the expression of the p21 protein was increased, the expression of proliferating cell nuclear antigen (pcna) protein decreased, and the expression of the p27 protein was unchanged as shown by western blot analyses. reg¦Ã plays a critical role in the cell cycle, proliferation and invasion of sw579 cells. the alteration of p21 and pcna proteins related to the down-regulation of reg¦Ã suggests that p21 and pcna participate in the process of reg¦Ã regulation of cell cycle progression and cell proliferation. thus, targeting reg¦Ã has a therapeutic potential in the management of pdtc patients.
%K reg¦Ã
%K pdtc
%K cell cycle
%K cell proliferation
%K sw579 cell line
%K thyroid carcinoma.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S0100-879X2012000500012&lng=en&nrm=iso&tlng=en