%0 Journal Article
%T Avalia£¿£¿o da matura£¿£¿o nuclear in vitro de o¨®citos de gatas dom¨¦sticas (Felis catus) pr¨¦-p¨²beres e p¨²beres
%A Lopes
%A Maria Denise
%A Uiechi
%A Edilson
%A Trinca
%A Luzia A.
%J Brazilian Journal of Veterinary Research and Animal Science
%D 2004
%I Universidade de S?o Paulo
%R 10.1590/S1413-95962004000400008
%X this study assessed the in vitro oocyte nuclear maturation in adult and young domestic cats. fifteen ovaries were used; 10 from adult females and 5 from young females. the oocytes collected by aspiration were quantified and classified. the oocytes classified as excellent and/or regular were grouped (10 oocyte /drop) in culture medium covered with mineral oil in disposable petri dishes. the oocyte were incubated at 38¡ãc and 5% de co2 for 48 hours and then washed with 0,4% hyaluronidase, fixed in methanol/acetic acid and stained with acetic orcein. evaluation of chromosomal configuration of oocytes matured in vitro showed 44,68% of oocyte in metaphase ii in the adult female group and 25,32% in the young donor group, showing that puberty influences oocyte capacity for in vitro development.
%K domestic cat
%K follicles
%K nuclear maturation.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1413-95962004000400008&lng=en&nrm=iso&tlng=en