%0 Journal Article
%T Interferon-gamma and interleukin-10 production by mononuclear cells from patients with advanced head and neck cancer
%A Conti-Freitas
%A Luiz C.
%A Foss-Freitas
%A Maria C.
%A Mamede
%A Rui C. M.
%A Foss
%A Norma T.
%J Clinics
%D 2012
%I Faculdade de Medicina / USP
%R 10.6061/clinics/2012(06)07
%X objective: this study aims to evaluate the production of interferon-gamma and interleukin-10 by stimulated peripheral blood mononuclear cells isolated from patients with supraglottic laryngeal cancer before and after surgical treatment. methods: fourteen patients with advanced supraglottic laryngeal cancer were studied. cultures of peripheral blood mononuclear cells isolated during the preoperative and late postoperative periods were stimulated with concanavalin a and bacille calmette-guerin, and the supernatant concentrations of interferon-gamma and interleukin-10 were measured. results: for non-stimulated cultures, the interferon-gamma levels produced by the preoperative period and the late postoperative period cultures were lower than the levels produced by the control group cultures. the interferon-gamma levels after stimulation with concanavalin a were higher in the late postoperative period cultures than in the preoperative evaluation cultures. stimulation with bacille calmette-guerin led to the production of similar levels of interferon-gamma and interleukin-10 by all cultures; thus, stimulation increased the levels of interferon-gamma produced by both the preoperative and postoperative cultures relative to the levels produced by the corresponding unstimulated cultures. conclusion: patients with advanced supraglottic laryngeal cancer exhibit an in vitro deficiency in interferongamma secretion by mononuclear cells. stimulated cells seem to recover this function during the postoperative period.
%K bcg
%K cytokines
%K interferon-£¿
%K interleukin-10
%K head and neck cancer.
%U http://www.scielo.br/scielo.php?script=sci_abstract&pid=S1807-59322012000600007&lng=en&nrm=iso&tlng=en