%0 Journal Article
%T Nuevo m¨¦todo para la cuantificaci¨®n de la actividad endoglucanasa basado en el complejo celulosa-rojo congo
%A Ortiz Moreno
%A Martha L.
%A Uribe - V¨¦lez
%A Daniel
%J ORINOQUIA
%D 2011
%I Universidad de los LLanos
%X the aim of this work was to establish a method for quantifying endoglucanase activity based on the depolymerisation of the congo red-polysaccharide complex. the mathematical viscosity and absorbance patternsforcongo red-carboxymethylcellulose(cmc) culture media were thus described. the inoculated media'sviscosity and absorbance were monitored for 8 daysto measure the impact of a cellulolytic fungus such as trichodermaviride on the media. enzymeconcentration was calculated by correlating reducing sugars'absorbance test results using3,5-dinitrosalicylic acid (dns) with loss of cmc culture mediaabsorbance. four cellulolytic deuteromycetesstrains were testedto assess the technique'sapplicability;it was found that usingcmc media absorbancelosswas effective for differentiatingstrains'endoglucanase activity. the congored-cmc method was faster and more sensitive than the dns method. this new method should thusbe appliedin screening assays when bioprospecting cellulolytic fungi in soil.
%K endoglucanase
%K carboxymethylcellulose
%K congo red
%K cellulolytic fungus.
%U http://www.scielo.org.co/scielo.php?script=sci_abstract&pid=S0121-37092011000100002&lng=en&nrm=iso&tlng=en