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中国生物工程杂志

ISSN Print: 1671-8135

ISSN Online:

主页: http://www.oaj.cas.cn/cn/journal_home.aspx?my_jid=1671-8135&journal_name=中国生物工程杂志

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Efficient Expression and Purification Technique of a Mutant of Human Interleukin 2
一种白细胞介素2基因变构体的高效表达与纯化工艺

Ming-jun Liu,
刘明军,王斌,宋旭霞,钱冬萌

Identification of tissues specific promoter of FLT-1 and detection its specific activity in transfected HUVEC
FLT-1启动子在血管内皮细胞中特异生物活性的检测及其意义

ZHANG Tao,
诸葛福媛,吕智美,郑宏庭,章涛,邓华聪

Removing Murine Embryonic Stem Cells from the differentiating cell culture by Using Magnetic Activated Cell Sorting
免疫磁珠分选降低分化体系中胚胎干细胞的比例

ZHU Wan-wan,DU Qing-an,WANG Shu-yan,XU Yan-ling,GUAN Yun-qian,ZHANG Yu,
朱宛宛,杜庆安,王淑艳,徐艳玲,关云谦,张愚

Molecular Cloning, Prokaryotic Expression and Biological Activity of α-2,6 Sialyltransferase
α-2,6唾液酸转移酶(ST6Gal1)的cDNA克隆、原核表达及生物活性研究

HOU Xiao-Qiang Xian-Zhu XIA,
侯小强,高玉伟,孙培录,冯娜,夏咸柱,杨松涛,段德明

Enhanced effects of BoNT/A DNA vaccines by electric pulses and bupivacaine
电脉冲和布吡卡因增强A型肉毒毒素DNA核酸疫苗的免疫效果

YU Yun-zhou,Li Na,WANG Shuang,YU Wei-yuan,SUN Zhi-wei,
余云舟,李娜,王双,俞炜源,孙志伟

Prokaryotic expression and acetylation assays of histone acetyltransferase PCAF
组蛋白乙酰转移酶PCAF在原核细胞的表达及其生物学活性检测

Wei-Zhong Li,
李卫中,张丹桂,曾俊,王革非,陈小璇,陈幼莹,李康生

Overexpression of α-synuclein Gene Caused Dopaminergic Neuron Damage in Substantia Nigra of Rats
AAV介导的α-synuclein基因过表达致多巴胺能神经元损伤-一种制作轻度帕金森病大鼠模型的新方法

LU Ling-ling,ZHOU Ai-xia,Yang Hui,
鲁玲玲,周爱霞,杨慧

The Knowledge Mapping Analysis on the Research of BioHydrogen
国际生物制氢相关研究的知识图谱分析

ZHAO Yong,
赵勇,孙成权,沙勇忠

多聚嘧啶序列结合蛋白（PTB）与HBV转录后调节元件（HPRE）结合抑制HBV表面抗原的基因表达

程丽英,张小花,李毅,蔡雪飞,胡源,黄爱龙,汤华

Activity study of specific bFGF-binding phage
bFGF特异性结合噬菌体的活性研究

YAN Qiu-xia,WU Xiao-ping,HUANG Hui-xian,NIE Chang-jun,NIE Yan-fang,KE Shi,XIAO Jian,LI Xiao-kun,
颜秋霞,吴晓萍,黄慧贤,聂昌君,聂燕芳,柯实,肖健,李校堃

Immobilization of an а-Cyclodextrin Glucanotransferase from Bacillus sp.602-1 and its Characterization
α-环状糊精葡萄糖基转移酶的固定化及其性质研究

chao ya-peng Shi-jun QIAN,
杨红艺,钞亚鹏,石家骥,解复红,薛智权,钱世钧

一种检测新型生物高分子材料聚γ-谷氨酸的新方法

张超,车程川,曲晓萌,杨革

Advances in Solid-state Fermentation of microbial Lipase
固态发酵生产微生物脂肪酶的研究进展

WANG Xiao-Feng,
汪小锋,闫云君

Anticancer Agents from Endophytic Fungi
植物内生真菌抗肿瘤药物研究进展

QIU Min,XIE Rui-sheng,SHI Yu,CHEN Hai-min,
邱敏,谢瑞生,史彧,陈海敏

Nucleic Acid Sequence-Based Amplification and Its Applications in Viral Diagnosis
NASBA(依赖核酸序列的扩增)及其在病毒检测中的应用

GAO Shan-dian,CHANG Hui-yun,CONG Guo-zheng,DU Jun-zheng,SHAO Jun-jun,LIN Tong,
高闪电,常惠芸,丛国正,独军政,邵军军,林彤

Construction of antimicrobial peptide Bactenecin 7 plasmid and its secretary
抗菌肽Bactenecin7重组质粒构建及其在乳酸菌的分泌表达和活性鉴定

LI Pu,WEN Yan-gan,LIU Jin-bo,YANG Xi-mei,ZHOU Jin-jing,TU Zhi-guang,
李朴,文阳安,刘靳波,扬细媚,周金敬,涂植光

Molecular Cloning, Co-expression and Characterization of dhaF and dhaG Genes Encoding Glycerol Dehydratase Reactivating Factor of Citrobacter freundii
弗氏柠檬酸菌甘油脱水酶激活因子基因的克隆、表达与功能鉴定

QI Xianghui Tian LIANG,
齐向辉,梁甜,曹博,罗兆飞,韦宇拓,杨登峰,黄日波

Cloning and Bioinformatics Analysis of PmNHX1 gene fromXinjiang Halophyte Plantago maritima
新疆盐生植物车前PmNHX1基因的克隆及生物信息学分析

Zhang Yu-Liang,
张雨良,张智俊,杨峰山,Mahesh,Kulye,袁辉,罗淑萍

Cloning and Characterization in Structure and Function of Fructan Biosynthesis Enzymes (FBEs) Gene in Plant
植物果聚糖合成酶基因克隆及特性分析

YIN Gui-xiang,SHE Mao-yun,GAO Xiang,WANG Jin,YE Xing-guo,
殷桂香,佘茂云,高翔,王瑾,叶兴国

Research Progress on Determination of Cellulase Activity and Gene Expression by Biosensor
生物传感器检测纤维素酶活性及基因表达的研究进展

YIN Juan,YUAN Xing-zhong,TANG Lin,
尹娟,袁兴中,汤琳

A Function of Targeting Fusion Protein XE-TNFαm2 to Lead Apoptosis in Cells Infected by HIV/SIV
靶向融合蛋白XE-TNFαm2导致受HIV/SIV感染细胞的凋亡

路金芝,陈伟京,杨晶,沈洋,蒋虹,王卫,李涛,吴钢,黄毅,佟薇,丛喆,魏强,王憬惺,卢圣栋

Construction of the Expression Vector with double report gene of EGFP-LacZ and its expression in vitro
EGFP-LacZ双报告基因真核表达载体的构建及体外表达

LIU Li Zheng Zhou Kejun Cai Yixiang Zhang HE Pai Fang Cao,
刘莉,周政,采克俊,张易祥,何湃,曹访

专稿2008年全球生物技术/转基因作物商业化发展态势——第一个十三年(1996-2008)

Clive,James

The Research Advance of Hair Follicle Stem Cell
毛囊干细胞研究进展

YUAN Lu,WANG Chun-sheng,AN Tie-zhu,
原璐,王春生,安铁洙

Methods for Regeneration of Five Kinds of SPR Sensor Chips and Their Applications
五种SPR传感芯片的再生制备及其应用

Hao Jiang Hongmin Zhou Leike Li Linjing Bai Cunxian Song Zhaofeng Luo,
欧惠超,姜浩,周宏敏,李磊珂,白林静,宋存先,罗昭锋

Drought Tolerance in Maize: An Emerging Reality
耐旱玉米

GO Edmeades,
G.O.Edmeades

Targeted Gene Mutagenesis and Replacement Mediated by Zinc Finger Nucleases
利用人工锌指蛋白核酸酶进行植物基因定点突变和置换

张余洋,张晓辉,张婵娟

Cloning of genes by genomic DNA Splicing for secretory IgA production
用基因组DNA剪接技术克隆SIgA相关基因

ZHANG Bao-zhong,AN Xiao-ping,ZHANG Xin,LIU Da-bin,SHAN Yun-zhu,ZHOU Yu-sen,TONG Yi-gang,
张宝中,安小平,张昕,刘大斌,单云竹,周育森,童贻刚

Identification of proteins interacting with hCLP46 from Human leukocyte cDNA Library using yeast two-hybrid system
酵母双杂交技术筛选与hCLP46蛋白相互作用的蛋白

张文娟,杜杰,常乃柏,张恬,王嵬,刘利新

Cell-based Screening of Phage Antibody Library
基于细胞的噬菌体抗体库筛选技术

YIN Chang-cheng,HUANG Hua-liang,YAN Xi-yun,
尹长城,黄华樑,阎锡蕴

Progress and insight of miRNA on hepatocellular carcinoma
miRNA在肝细胞癌中的研究进展和展望

戚鹏,高春芳

Study on the separation and purification technology of catalpol from Rehmannia by macroporous adsorption resins
大孔吸附树脂分离纯化地黄中梓醇工艺的研究

ZHANG Xiu-li,WANG Jing-yun,JIANG Bo,CHANG Yan,AN Li-jia,
张秀丽,王静云,姜波,安利佳

Cloning and Prokaryotic Expression of Human Recombinant Calreticulin
重组人钙网蛋白的克隆与原核表达

CAO Chun-yu,HAN Yu,WANG Yan-lin,
曹春雨,韩钰,王艳林

Regulation of gene expression profile by Twist in murine breast cancer cell
Twist对小鼠乳腺癌细胞基因表达谱的调控研究

Yu-chao GU,
杨静,顾玉超,于文功

Soluble Expression, Purification, DNA Binding Activity of Mouse TAp63γ and Two Deltion Mutants Fusion Proteins
小鼠TAp63γ及两种突变体的原核表达、纯化和DNA结合活性

Yan Jun-Xi,
严君喜,黄新河,杜林方,Zhi-Xiong,Jim,Xiao

Proteomic analysis of Splenolymphocyte in rat with cerebral hemorrhage and cerebral ischemia
脑出血与脑缺血动物模型脾淋巴细胞的蛋白质组学研究

XIONG Xin-Gui Qing-hua LIANG Jiang CHEN Nian-ju ZENG Jian-Gang OU Rong FAN,
熊新贵,梁清华,陈疆,曾年菊,区健刚,范荣

A Promising Biological Detection Technology-Biosensor
极具有应用前景的生物学检测技术-生物传感器

HAN Xue-qing,YANG Ze-xiao,LIN Xiang-mei,
韩雪清,杨泽晓,林祥梅

Cloning and Expression Analysis of two Endosperm Promoters in Rice
两个水稻胚乳启动子的克隆及表达分析

CUI Yong-lan,ZHONG Xiao-li,ZHANG Yong-ming,YANG Zhong-nan,
崔永兰,钟晓丽,张永明,杨仲南

The expression of antibacterial peptide CM4 in Escherichia coli fused with human soluble B lymphocyte stimulator activing factor
抗菌肽CM4与人可溶性B淋巴细胞因子hsBAFF在大肠杆菌中的融合表达

LIU Hai-feng,WANG Xiao-hua,LIU Ping,ZHANG Shuang-quan,
刘海峰,王小花,刘平,张双全

The preparation of the recombinant Fusion protein Human Retinal Pigment Epithelium-Derived Factor and the analysis of its angiogenesis activity
重组人色素上皮细胞衍生因子融合蛋白的制备及其抑制新生血管

王亚妮,刘先宁,朱娟莉,安娜,朱秀萍

Chemical Modification and Active Sites of β-Fructosyl-transferase from Aspergillus oryzae GX0011
米曲霉GX0011β-果糖基转移酶的化学修饰及活性中心研究

Qin Yi-min,TANG Jiang-tao,WEI Yuan-an,YAO Ping-jia,Liang Jin-tian,
覃益民,唐江涛,魏远安,姚评佳,梁锦添

Immobilization of β-glucosidase with Nano SiO2 and Its Application in Soybean Isoflavone Hydrolysis in Two-phase-system
纳米SiO2固定化β-葡萄糖苷酶及其在双相体系中水解大豆异黄酮的工艺研究

HUANG Zhe Tao ZHANG Zhang-lin LIN,
黄哲,张涛,林章凛

Optimization of Technological Process for Synthesizing Biodiesel from Kosteletzkya virginica Oil
海滨锦葵油制备生物柴油工艺条件优化

YANG Qing-li,YU Shan-lin,QIN Song,
杨庆利,禹山林,秦松

Progress on the Study of Sumoylation
蛋白质SUMO化修饰研究进展

韦玮 张浩 毛建平,叶棋浓

Prokaryotic expression and functional study of HIV-1 envelope glycoprotein gp41 helical bundle
HIV-1跨膜蛋白gp41螺旋结构的原核表达及功能研究

LIU Bin,Shao-hui CHENG,
刘斌,何红秋,程绍辉,陈慰祖,王存新

Regulation of Lipids Synthesis in Transgenic Escherichia coli by Inserting Cyanobacterial Sense and Antisense pepcA Gene
蓝藻正反义pepcA基因导入对大肠杆菌中脂类合成的调控

HOU Li-jun,SHI Ding-ji,CAI Ze-fu,SONG Dong-hui,WANG Xue-kui,
侯李君,施定基,蔡泽富,宋东辉,王学魁

Localization of Arabidopsis VHA-c3 Promoter with GUS-Fusion
拟南芥VHA-c3启动子的GUS基因融合表达

郭荣起,苏杰,王福慧,徐萍,伊莉佳,姜树原,王瑞刚

Studies on the Purification and Characterization of an α-cyclodextrin Glucanotransferase
一种α-环糊精葡萄糖基转移酶的纯化及性质研究

Jun-ying WANG chao ya-peng Dong-ming Guan Shi-jun QIAN,
王俊英,钞亚鹏,关东明,钱世钧

Research Progress in Succinic Acid Fermentation of Actinobacillus succinogenes
产琥珀酸放线杆菌发酵生产琥珀酸的研究进展

张玉秀,张云剑,李强

Progresses on Biosynthesis of Long Chain Polyunsaturated Fatty Acids in Plant
植物合成长链多不饱和脂肪酸研究进展

LI Hui-zhen,ZHANG Zhi-jun,
李会珍,张志军

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