Detection of Neospora sp. antibodies in cart horses from urban areas of Curitiba, Southern Brazil

neospora caninum is a protozoan parasite which affects dogs as definitive hosts and several mammalian species as intermediate hosts mainly causing abortions and central nervous system disorders. the reemerging population of cart horses for carrying recycling material in urban areas of major cities in brazil may have an impact on disease spreading, and these animals may be used as sentinels for environmental surveillance. thus, the present study investigated the frequency of neospora sp. antibodies in cart horses from curitiba and surrounding areas, paraná state, southern brazil. igg antibodies against neospora sp. were detected using indirect fluorescence antibody test (ifat), and titers equal to or higher than 1:50 were considered reactive. of all samples, 14/97 (14.4%) were positive: 2/29 (6.9%) were younger than 5; 5/26 (19.2%) between 6 and 9; and 6/31 (19.4%) older than 10 years of age. one of the 11 animals with unknown age was positive (9.1%). cart horses are likely to be more exposed to dog feces and to neospora sp. oocyst contamination in urban settings and a lower frequency of disease in dogs may have a negative impact on horse infection risk in these areas.