Histological and histochemical characterization of secretory cells of the male copulatory organs of Girardia anderlani (Platyhelminthes: Tricladida: Paludicola)

glands of the reproductive system are important for taxonomical identification of flatworms. we studied the histology and histochemical characteristics of the glands of the male copulatory apparatus in girardia anderlani (kawakatsu & hauser, 1983). specimens were fixed in reproductive state, i. e. during and following copulation at four, eight, 12 and 16 hours intervals. secretory cells were distinguished on the basis of secretion morphology and their staining properties, using trichrome methods and histochemical reactions. twelve secretory cell types and five main types of secretions were identified in the male copulatory apparatus: glycoproteic with and without tryptophan; glycosaminoglycidic; neutral mucopolysaccharidic; and proteic. compared to other girardia species, more diverse types of secretory cells comprise the glands of the male reproductive system. histophysiological comparative studies of species of girardia, in a reproductive state, are necessary to characterize the various regions of the copulatory apparatus as well as to understand the physiology of reproduction.