EFECTO DE ALCOHOLES Y POLIOLES SOBRE LA ESTABILIDAD ESTRUCTURAL DE LA β-LACTOGLOBULLNA EN SOLUCIóN ACUOSA

in this work we present a systematic study of the effect of the increase of oh groups in alcohols and polyols of four carbon atoms on the conformational properties of β-lactoglobulin. the changes in the behavior of the aqueous solution of the protein by the addition of 1-butanol, 1,2-butanediol, 1,2,4-butanetriol y 1,2,3,4-butano-tetrol were determined by uv, far and near uv cd spectra and fluorescence spectroscopy at 298.15k. the results show that the largest modification ofprotein structure is due to butanol and the effect decreases as the number of oh groups increase.