|
Confirmación de la presencia en Cuba del virus linfotrópico tipo I de las células T humanas mediante la reacción en cadena de la polimerasaKeywords: htlv-i [isolation & purification], htlv-i infections [blood], polymerase chain reaction [methods], oligonucleotides [diagnostic use], reagent kits, diagnostic, cuba. Abstract: the first cases diagnoses in cuba as htlv-i/ii seropositives (human t lymphotropic virus) were studied by polymerase chain reaction aimed at differentiating the type of virus causing the infection. 3 kits of primer oligonucleotides were used and the amplification products were detected by hybridization with specific oligoprobes. 100 % of the cases were htlv-i positives. no htlv-ii positivity was found. it was confirmed the presence in cuba of this retrovirus, eventhough the seroprevalence is low if it is taken into consideration that the caribbean is an endemic zone for htlv-1.
|