Proliferación de células madres mesenquimales obtenidas de tejido gingival humano sobre una matriz de quitosano: estudio in vitro

aim: the purpose of this study was to assess the proliferation of mesenchymal stem cells (mscs) from human gingival tissue on chitosan matrix. methods: experimental study in vitro. gingival connective tissue samples were obtained from healthy volunteers from the maxillary tuberosity. the explants were minced and cultured on tissue culture dishes. msc were characterized by flow cytometry using markers for cd34, cd45, cd73, cd90, cd105 and for differentiation into, adipogenic, osteogenic and chondrogenic lineages. the tissue differentiated was analyzed with light microscopy and evaluated by culture staining using oil red, alizarin red y safranina o respectively. msc from passage 5 were cultured with chitosan scaffold. proliferation of msc was analyzed with light microscopy and crystal violet staining. results: mscs were obtained from gingival explants, and developed the standard of the morphologic and phenotypic characterization as a stem cell. the msc adopted a fibroblastoid morphology, adherence to plastic, confluence of 80% and over 90% were consistently positive for cd90, cd105, cd73 markers and under 10% were negative for hematopoietic markers cd34 and cd45 by flow cytometry analysis. the msc cultured in presence of chitosan matrix proliferated, however complete medium, it was dissolved forming a gel structure. we also observed that at higher concentrations of chitosan, msc has less density and growth. chitosan matrix in presence of cell culture medium loses physical properties, dissolving and forming a non-transportable gel. conclusions: despite the existence of proliferation of mscs from human gingival tissue with chitosan matrix, its ability to act as a cell carrier and scaffold is deficient, since its physical properties are altered, dissolving and forming a non-transportable gel in contact with cell culture medium.