Manejo de Plántulas de Mango (Mangifera indica L.) Obtenidas in vitro para Estudios de Sanidad Vegetal

in vitro mango seedlings free of external and seed-transmitted contaminants were obtained from immature embryos. then, they were transplanted to soil using only sterilized materials. mango embryos 45-60 days old were plated in modified b5 medium; and after seedlings attained 10 cm height, they were transplanted to soil + expanded perlite in specially designed plastic containers. plants were irrigated with nutritive universal solution. during the in vitro stage 10% contamination with penicillium, aspergillus, xanthomonas and cladosporium was observed. contamination in plastic containers gradually reached 15% after five months. cladosporium, alternaria, aspergillus, rhizopus, and fusarium were identified in the substrate, whereas helminthosporium, curvularia, penicillium, and syncephalastrum appeared in the plastic containers.