Mutaciones asociadas con resistencia a rifampicina o isoniazida en aislamientos clínicos de M. tuberculosis de Sonora, México

objective: to perform the analysis of specific regions of the major genes associated with resistance to isoniazid or rifampin. materials and methods: twenty two m. tuberculosis strains, isolated from human samples obtained in sonora, mexico. specific primers for hotspots of the rpob, katg, inha genes and the ahpc-oxyr intergenic region were used. the purified pcr products were sequenced. results: mutations in the promoter of inha, the ahpc-oxyr region, and codon 315 of katg and in 451 or 456 codons of rpob, were identified. conclusions: detection of mutations not previously reported requires further genotypic analysis of mycobacterium tuberculosis isolates in sonora.