Diversity and characterization of ramie-degumming strains

ramie (boehmeria nivea and boehmeria tenacissima) is a widely used fiber crop. traditional water retting or chemical boiling method performed in order to extract ramie fiber seriously pollute the environment and severely damage the fiber, so biological method is the general trend of the fiber-extracting industry. some strains (687), involving 26 genera and 43 species, were collected from the three samples, which produce hydrolyzed circles in the selective culture medium in order to detect the degumming effect and to compare the enzyme activity. among these strains, 13 of them did not produce cellulase and had a ramie decreasing weight rate above 25 %, which were regarded as efficient ramie-degumming strains named from r1 to r13. r1 to r13 belonged to amycolata autotrobutylicun, bacillus subtilis, clostridium acetobutylicum, bacillus subtilis, rhizobium leguminosarum, bacteroides finegoldii, streptomyces lividans, bacillus amyloliquefaciens, clostridium acetobutylicum, pseudomonas brassicacearum, bacillus pumilus, bacillus licheniformis, pectobacterium wasabiae respectively. bacteroides sp., rhizobium sp. and pseudomonas sp. were firstly reported to be used in ramie-degumming. at the same time, the pectinase was the key enzyme in the ramie-degumming process.