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AMB Express 2013
Method to quantify live and dead cells in multi-species oral biofilm by real-time PCR with propidium monoazideKeywords: Oral biofilm, Real-time PCR, Propidium monoazide (PMA), Antiseptic Abstract: In short, we standardised the conditions for using qPCR-PMA in 5 oral bacterial species and proved its usefulness for quantification of live and dead cells in multi-species oral biofilms formed in vitro, after use of an antiseptic.
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