Assessment of Microbiological Quality of Water Wells in Rural Properties of the City of West of Santa Catarina, Brazil

Due to the large consumption of water wells and springs in rural properties in the far west region of Santa Catarina and illnesses related to consumption of contaminated water, this study was to evaluate the microbiological quality of water wells in rural properties in the far west of Santa Catarina, Brazil. Thus, this study can be used as a tool to discuss the current state of the wells that supply farms in the far west of Santa Catarina, since it is necessary to introduce orientation programs for consumers of untreated , for the most part unaware of these basic methods for maintaining the quality of drinking water. The research was conducted with Company of Agricultural Research and Rural Extension of Santa Catarina SA (EPAGRI) of Sao Miguel do Oeste, SC. We collected samples from 70 wells from 07 city in the region west of Santa Catarina from May 2011 to February 2012, and collected three samples per well evaluated in different periods. Samples were collected by technical EPAGRI of each municipality participating in the study and subsequently transported and analyzed in the Research Laboratory and Diagnostic Microbiology of the University of West of Santa Catarina. Towards assess the quality of water was used for faecal technique for Multiple Tube Fermentation and heterotrophic bacterial count technique pour plate with standard agar for count established by the Normative Instruction No. 62, August 26, 2003 the Ministry of Agriculture , Livestock and Supply. In the laboratory, were applied to wells of a user questionnaire for their perception of water quality. Of the 70 wells analysed, 67 (95.71%) were considered unsuitable for human consumption, according Decree in 2914 to 12 December 2011 the ministry of health. The average amount of total coliform samples found unsuitable (199 samples from 67 wells) was 366.09 MPN / 100 ml, ranging from 265.98 to 527.1 MPN /100 mL. Since the average amount of faecal coliform found in these samples (those considered unfit) was 62. 34 MPN /100 mL ranging from 12.73 to 105.5 MPN /100 mL. For the average score heterotrophic microorganisms in the samples was unsuitable 1386 CFU / ml, ranging from 836 to 2234 CFU / ml. Although the data found in laboratory tests, the questionnaires showed that 82.88% of owners who use water classified as good or excellent quality, and less than half (42.84%) filter or boil water before consuming it .Thus, we can see that it is extremely important that preventive measures are taken for the preservation of the sources and improving water quality in the region, since most water wells are unfit for human consumption and which further aggravates these results is the users' perception about the quality, which contributes to the lack of concern with basic care to avoid such a problem.