Exigent of Micro Algae for the Enrichment of Artemia salina

The nauplii of most strains of Artemia are lacking in certain biochemical elements required by some species of fish. The cysts from commercial traders test positve for DHA, however they are extremely difficult to obtain and very expensive. Most strains also have a low energy to protein ratio. These deficiencies can be remedied by an enrichment process. Artemia salina was enriched with three different algal species (Nannochloropsis salina, Chlorella salina and Spirulina subsalsa) and commercially available media A1 DHA SELCO media. After enrichment process, proximate analysis was carried out by Lowry s (protein), Anthrone (carbohydrate) and Phenol-Chloroform Method (lipid). Nannochloropsis salina and C. salina enriched Artemia be full of high lipid which is the nutrient content of both algae. Spirulina subsalsa enriched Artemia showed the high protein which is the nutrient content of S. subsalsa. Chlorella salina and N. salina enriched Artemia illustrated that good result in both protein and lipid. Concentration of the Artemia biomass was directly proportional with hours of enrichment. From this study, researchers can use algae (N. salina, C. salina and S. subsalsa) as a enrichment media instead of commercial A1 DHA SELCO media which give same nutrition profile to Artemia.