Effects of Enhanced Digestive Enzymes Activity with Starch and Glycogen During Rotifer Brachionus rotundiformis Culture

The purpose of this study was to identify the digestive enzymes activity of rotifer Brachionus rotundiformis enhanced by poly-saccharides added in medium and lipid classes and fatty acids changed after their enrichment. Population growth of rotifer showed significantly higher density in additive trials (starch and glycogen) than non-addition (control) (p<0.05). The activity assayed significantly higher amylase and Total Alkaline Protease (TAP) in additive trials than control after culture (p<0.05). But, TG-lipase activity was significantly higher starch trial only than control after culture and enrichment (p<0.05). Total lipid contents analyzed to significantly higher 9.39% in starch trial only than control after enrichment (p<0.05). Free fatty acid and sterol contents showed significantly higher in additive trials than control after culture and enrichment (p<0.05). Unsaturated fatty acid index (UI) analyzed to significantly higher 227.6-236.6 in additive trials than 205.1 of control (p<0.05). DHA content was higher 15.3-16.7% in additive trials than 13.1% of control after enrichment (p<0.05). Therefore, researchers saw a possibility to raise sterol content that precursor of useful ingredient as several enzyme and hormone. And researchers could be confirmed the lipid utility increased of fish larvae by raising UI by use of starch and glycogen used in the study.