Molecular Cloning and the Developmental Patterns of the PLIN Gene in Goose

PLIN plays a central role in the regulation of adipocyte metabolism which drives triacylglycerol storage in adipocytes and requires to hormonally stimulated lipolysis by cellular lipases. However, there is no report about the molecular structure and the expression profile of PLIN in goose. In this study, we cloned the goose PLIN gene and predicted bioinformatics. Quantitative Real Time PCR (qRT-PCR) assays were developed for accurate measurement of PLIN mRNA levels in Zhejiang White goose (ZW) and Landes goose different tissues from different ages (0-9 weeks). The results showed that goose PLIN cDNA sequence encoded an open reading frame of 527 Amino Acids (AA). The molecular phylogenetic tree among species were constructed that the mammal grouped together, Bombyx mori became another branch while the goose and chicken became another branch. It was found that PLIN mRNA were highly expressed in adipose tissues and to a lesser extent in cardiac and skeletal muscle and the difference is extreme significant between the fat and other tissues (p<0.01) yet, no expression in liver. In addition, there was a significantly age-related and breed-related change in fat tissues (p<0.05) and PLIN mRNA expression in ZW fat higher than that of Landes goose. All these results showed that the expression of PLIN mRNA in adipose tissues exhibited specific developmental changes, age-related and breeding patterns. The patterns of PLIN gene suggested that it played an important role in geese fat development. What s more, further study is needed to reconfirm its function in a large population and in other breeds with different genetics background.