Changes of Fresh Weight, Contents of Dry Mass, Soluble Carbohydrates and Soluble Proteins During Development of Loquat (Eriobotrya japonica Lindl.) Anther-Derived Embryos

Histological studies revealed that the anther-derived embryos of loquat developed through the typical globular, heart, torpedo and cotyledon stages. The changes of fresh weight, contents of dry mass, soluble carbohydrates and soluble proteins were studied during the development of anther-derived embryos of Loquat. The fresh weight increased with the anther-derived embryos developing. With the largest increment from the torpedo stage to cotyledon stage, the dry mass trended to increase at a low level, which was the lowest at the heart stage. In the embryogenic callus formed from anther-derived embryo, the contents of soluble carbohydrate were the most. The globular anther-derived embryos have a higher level of contents of soluble carbohydrates following in the embryogenic callus. But the embryos at globular, heart, torpedo and cotyledon stages kept a low-level contents of soluble carbohydrates and changed with a small extent. The contents of soluble proteins were high in embryogenic callus formed from anther-derived embryo and globular embryos. But it fall with different level at all next stages.