ELISA OF HUMAN PLACENTA TYPE GLUTATHIONE S-TRANSFERASE AND ITS APPLICATION IN THE DIAGNOSIS OF HEPATOCARCINOMA
人胎盘型谷胱甘肽S-转移酶的ELISA及用于肝癌的诊断

从人体胎盘提纯GST-π,制备兔抗GST-π血清,纯化其IgG,并降解成Fab′片断,通过SMCC与HRP交联成Fab′-HRP,用于夹心ELISA以测定GST-π,灵敏度可达11pg,超过国外的放射免疫法。用此法测定正常人血清GST-π为1.06±0.94ng/ml,原发性肝癌血清较正常高20倍以上。阳性率达90％左右。