Isolation and identification and antibiotic susceptibility test of pathogenic bacteria strain from hybridized prussian carp
异育银鲫致病性嗜水气单胞菌的分离鉴定与药敏特性研究

The bacteria were isolated and identified from hemorrhage diseased C.giblio and drug sensitive tests were performed in order to develop effective control and prevention measures.Two dominant bacterial strains designated as WJ11-3, WJ11-4 were isolated from the liver, spleen, kidney,gills of the sick C.giblio. The pathogens were identifid by morphological observation, biochemical test and 16S rRNA sequence analysis. The susceptibility test was done by disc diffusion, The pathogens were identified as Aeromonas hydrophila. They could lead to the death of C.giblio, and could reproduce the diseased symptoms as occurred in wild. The antibiotic sensitivity analysis showed that WJ11-3 was resistant to oxacillin, nitrofurantoin and novobiocin. 28 kinds of drugs were sensitive to WJ11-3, which includes cefuroxime, nitrofurantoin, doxycline, etc. 28 kinds of drugs were sensitive to WJ11-4, which includes florfenicol, sinomin, nalidixic acid, lomefloxacin, etc. The above mentioned antibiotics may be used to control and prevent hemorrhage diseased in C.giblio caused by Aeromonas hydrophila in a farm of Yancheng, Jiangsu.