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植物学报  2011 

Optimization Process and Method of 2-D Electrophoresis for Rice Proteomics
水稻蛋白质组双向电泳优化流程及方法

Keywords: proteomics,rice,2-D electrophoresis
蛋白质组学
,水稻,双向电泳

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Abstract:

2-D electrophoresis is a powerful tool for analyzing protein mixtures. It has been widely used in proteome research. Rice is an important food crop, and proteomic research into rice has been extensive. Because of the technical complexity and time constraints, performing the procedure accurately has been difficult. This paper describes an optimization process for 2-D electrophoresis of various organs of rice. This method can help researchers optimize experimental conditions with better accuracy.

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