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Antibodies  2012 

Quality Control System for Beer Developed with Monoclonal Antibodies Specific to Barley Lipid Transfer Protein

DOI: 10.3390/antib1030259

Keywords: lipid transfer protein, beer, ELISA, food allergy, monoclonal antibody

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Abstract:

Non-specific lipid transfer protein (LTP) in barley grain reacted with the IgE in sera drawn from food allergy patients. A sandwich-type of enzyme-linked immunosorbent assay (ELISA) was developed with mouse monoclonal antibodies raised against LTP purified with barley flour. This ELISA showed a practical working range of 0.3–3 ng/mL and no cross-reactivity with wheat, adlay and rye. Using this ELISA, LTP was determined in several types of barley-foods, including fermented foods such as malt vinegar, barley-malt miso and beer. LTP content in beer of the same kind was approximately constant, even if manufacturing factory and production days were different. Not only as a factor of foam formation and stability but also as an allergen, controlling and monitoring of LTP in beer should be considered. Taken together, our LTP-detecting ELISA can be proposed as an appropriate system for the quality control of beer.

References

[1]  Kader, J.C. Lipid-transfer proteins in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 1996, 47, 627–654, doi:10.1146/annurev.arplant.47.1.627.
[2]  Douliez, J.P.; Michon, T.; Elmorjani, K.; Marion, D. Structure, biological and technological functions of lipid transfer protein and indolines, the major lipid binding protein from cereal kernels. J. Cereal Sci. 2000, 32, 1–20, doi:10.1006/jcrs.2000.0315.
[3]  Garcia-Olmedo, F.; Molina, A.; Segura, A.; Moreno, M. The defensive role of nonspecific lipid-transfer proteins in plants. Trends Microbiol. 1995, 3, 72–74, doi:10.1016/S0966-842X(00)88879-4.
[4]  Kader, J.C. Lipid-transfer proteins: A puzzling family of plant proteins. Trends Plant Sci. Biol. 1997, 2, 66–70, doi:10.1016/S1360-1385(97)82565-4.
[5]  Hincha, D.K. Cryoprotectin: A plant lipid- transfer protein homologue that stabilized membranes during freezing. Philos. Trans. R. Soc. Lond. B. Biol. Sci. 2002, 357, 909–919, doi:10.1098/rstb.2002.1079.
[6]  Gaudet, D.; Laroche, A.; Frick, M.; Huel, R.; Puchalski, B. Cold induced expression of plant defensin and lipid transfer protein transcripts in winter wheat. Physiol. Plant 2003, 117, 195–205, doi:10.1034/j.1399-3054.2003.00041.x.
[7]  Bubier, J.; Schlappi, M. Cold induction of EARLI1, a putative Arabidopsis lipid transfer protein, is light and calcium dependent. Plant Cell Environ. 2004, 27, 929–936, doi:10.1111/j.1365-3040.2004.01198.x.
[8]  Gorjanovi?, S.; Su?njevi?, D.; Beljanski, M.; Ostoji?, S.; Gorjanovi?, R.; Vrvi?, M.; Hranisavljevi?, J. Effects of lipid-transfer protein from malting barley grain on brewers yeast fermentation. J. Inst. Brew. 2004, 110, 297–302, doi:10.1002/j.2050-0416.2004.tb00624.x.
[9]  Sohal, A.K.; Pallas, J.A.; Jenkins, G.I. The promoter of a Brassica napus lipid transfer protein gene is active in a range of tissues and simulated by light and viral infection in transgenic Arabidopsis. Plant Mol. Biol. 1999, 41, 75–87, doi:10.1023/A:1006232700835.
[10]  Park, C.J.; Shin, R.; Park, J.M.; Lee, G.J.; You, J.S.; Paek, K.H. Induction of pepper cDNA encoding a lipid- transfer protein during the resistance response to tobacco mosaic virus. Plant Mol. Biol. 2002, 48, 243–254, doi:10.1023/A:1013383329361.
[11]  Zasloff, M. Antimicrobial peptides of multicellular organisms. Nature 2002, 415, 389–395, doi:10.1038/415389a.
[12]  Van Loon, L.; Van Strien, E. The families of pathogenesis-related proteins, their activities, and comparative analysis ofPR-1 type proteins. Physiol. Mol. Plant Pathol. 1999, 55, 85–97, doi:10.1006/pmpp.1999.0213.
[13]  Sanchez-Monge, R.; Lombardero, M.; Garcia-Selles, F. J.; Barber, D.; Salcedo, G. Lipid-transfer proteins are relevant allergen in fruit allergy. J. Allergy Clin. Immunol. 1999, 103, 514–519, doi:10.1016/S0091-6749(99)70479-3.
[14]  Palacin, A.; Cumplido, J.; Figueroa, J.; Ahrazem, O.; sanchez-Monge, R.; Carrillo, T.; Salcedo, G.; Blanco, C. Cabbage lipid transfer protein Bra o 3 is major allergen responsible for cross-reactivity between plant foods and pollens. J. Allergy Clin. Immunol. 2006, 117, 1423–1429, doi:10.1016/j.jaci.2006.01.026.
[15]  Curioni, A.; Santucci, B.; Cristaudo, A.; Canistraci, C.; Pietravalle, M.; Simonato, B.; Giannattasio, M. Urticaria from beer: An immediate hypersensitivity reaction due to a 10 kDa protein derived from barley. Clin. Exp. Allergy 1999, 29, 407–413, doi:10.1046/j.1365-2222.1999.00491.x.
[16]  Pastrorello, E.A.; Farioli, L.; Pravettoni, V.; Robino, A.M.; Scibilia, J.; Fortunato, D.; Conti, A.; Borgonovo, L.; Bengtsson, A.; Ortolani, C. Lipid transfer protein and vicilin are important walnut allergens in patients not allergic to pollen. J. Allergy Clin. Immunol. 2004, 114, 908–914, doi:10.1016/j.jaci.2004.06.020.
[17]  Hiller, K.M.; Lubahan, B.C.; Klapper, D.G. Cloning and expression of ragweed allergen Amb a 6, Scand. J. Immunol. 1998, 48, 26–36.
[18]  Charvolin, D.; Douliez, J.P.; Marion, D.; Cohen-Addad, C.; Pebay-Peyroula, E. The crystal structure of a wheat nonspecific lipid transfer protein (ns-LTP1) complexed with two molecules of phospholipids at 2.1 ? resolution. Eur. J. Biochem. 1999, 264, 562–568, doi:10.1046/j.1432-1327.1999.00667.x.
[19]  Lerche, M.H.; Poulsen, F.M. Solution structure of barley lipid transfer protein complexed with palmitate. Two different binding modes of palmitate in the homologous maize and different nonspecific lipid transfer protein. Protein Sci. 1998, 7, 2490–2498, doi:10.1002/pro.5560071202.
[20]  Salcedo, G.; Sanchez-Monge, R.; Diaz-Perales, A.; Garcia-Casado, G.; Barber, D. Plant non-specific lipid transfer proteins as food and pollen allergens. Clin. Exp. Allergy 2004, 34, 1336–1341, doi:10.1111/j.1365-2222.2004.02018.x.
[21]  Salcedo, G.; Diaz-Perales, A.; Sanchez-Monge, R. Fruits allergy: Plant defence protein as noval potential panallergen. Clin. Exp. Allergy 1999, 29, 1158–1160, doi:10.1046/j.1365-2222.1999.00629.x.
[22]  Lindroff-Larsen, K.; Winther, J.R. Surprisingly high stability of barley lipid transfer protein, LTP1, towards denaturant, heat and proteases. FEBS Lett. 2001, 488, 145–148, doi:10.1016/S0014-5793(00)02424-8.
[23]  Van Nierop, S.N.E.; Evans, D.E.; Axcel, B.C.; Cantrell, I.C.; Rautenbach, M. Impact of different wort boiling temperatures on the beer foam stabilizing properties of lipid transfer protein 1. J. Agric. Food Chem. 2004, 52, 3120–3129, doi:10.1021/jf035125c.
[24]  Garcia-Casado, G.; Crespo, J.F.; Rodriguez, J.; Salcedo, G. Isolation and charasterization of barley lipid transfer protein and protein Z as beer allergens. J. Allergy Clin. Immunol. 2001, 108, 647–649, doi:10.1067/mai.2001.118793.
[25]  Quercia, O.; Zoccatelli, G.; Stefanini, G.F.; Mistrello, G.; Amato, S.; Bolla, M.; Emiliani, F.; Asero, R. Allergy to beer in LTP-sensitized patients: Beers are not all the same. Allergy 2012, 67, 1186–1189, doi:10.1111/j.1398-9995.2012.02872.x.
[26]  Akiyama, H.; Imai, T.; Ebisawa, M. Japan food allergen labeling regulation—History and Evaluation. In Advances in Food and Nutrition Research; Taylor, S., Ed.; Academic Press: Burlington, MA, USA, 2011; Volume 62, pp. 139–171.
[27]  Lowry, O.H.; Rosebrough, N.J.; Farr, A.L.; Randall, R.J. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 1951, 193, 265–275.
[28]  Van Paridon, P. A.; Gadella, T.W.J., Jr.; Wirtz, K.W.A. The effect ofpolyphosphoinositides and phosphatidic acid on the phosphatidylinositol transfer protein from bovine brain: Kinetic study. Biochim. Biophys. Acta 1988, 943, 76–86, doi:10.1016/0005-2736(88)90348-3.
[29]  Lusk, L.T.; Navarro, A.L.; Goldstein, H.; Wagner, R.J.; Ryder, D.S. Monoclonal antibodies for assaying lipid transfer proteins. U.S. Patent 6423546, 23 July 2002.
[30]  Hirose, J.; Kitabatake, N.; Kimura, A.; Narita, H. Recognition of native and/or thermally induced denatured forms of the major food allergen, ovomucoid, by human IgE and mouse monoclonal IgG antibodies. Biosci. Biotechnol. Biochem. 2004, 68, 2490–2497, doi:10.1271/bbb.68.2490.
[31]  Hirose, J.; Murakami-Yamaguchi, Y.; Ikeda, M.; Kitabatake, N.; Narita, H. Oligoclonal enzyme-linked immunosorbent assay capable of determining the major food allergen, ovomucoid, irrespective of the degree of heat denaturation. Cytotechnology 2005, 47, 145–149.
[32]  Honjoh, T.; Muraoka, S.; Mamekoshi, S.; Sakai, M. Detection methods for allergic substances in foods by enzyme linked immunesorbent assay. Foods Food Ingredients J. Jpn. 2002, 206, 13–22.
[33]  Murakami-Yamaguchi, Y.; Hirose, J.; Honjoh, T.; Narita, H. Evaluation of wheat content with monoclonal antibodies against lipid transfer protein. J. Jpn. Soc. Food Ssi. Technol. 2009, 56, 16–24.
[34]  Laemmli, U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227, 680–685, doi:10.1038/227680a0.
[35]  Kohler, G.; Milstein, C. Continuous cultures of fused cells secreting antibody ofpredefined specificity. Nature 1975, 256, 495–497, doi:10.1038/256495a0.
[36]  Nakane, P.K.; Kawaoi, A. Perioxidase-labelled antibody. New method of conjugation. J. Histochem. Cytochem. 1974, 22, 1084–1091, doi:10.1177/22.12.1084.

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