Effects of different antioxidants on cryopreservation of Jinhua boar sperm
不同抗氧化剂对金华猪精液冷冻保存效果的影响

To compare the effects of different antioxidant concentrations of glutathione (GSH), green tea polyphenols (GTPs), vitamin E (V_E) and superoxide dismutase (SOD) on cryopreservation of Jinhua boar sperm, freezing extenders were supplemented with the GSH (0.125, 0.25 and 0.5 mmol·L～(-1)), GTPs (10, 15 and 20 mmol·L～(-1)), V_E (1, 2 and 4 mg·mL～(-1)) and SOD (1000, 2000 and 4000 IU), respectively. The boar sperms were collected and frozen with extenders containing above antioxidants. Sperm motility after 4 ℃cooling, sperm motility, membrane integrity and acrosome integrity after thawing were examined in four treatment groups. The results show that sperm motility after 4 ℃cooling, sperm motility and membrane integrity after thawing were increased significantly by supplemented boar freezing extenders with 15 mmol·L～(-1) GTPs, 2 mg·mL～(-1) V_E or 4000 IU SOD, respectively (P< 0.05).