Detection of Gallibacterium anatis in layers by PCR and their histopathological observation in natural case
鸡卡氏杆菌的PCR检测及自然病例的组织病理学观察

In order to observe the histopathological changes of the layer oviduct cysts infected with Gallibacterium anatis,target genes were amplified by PCR from genome DNA extracted from lung,trachea,oviduct and ovary of nine birds infected naturally,using genus-specificity primes of Gallibacterium anatis,and the Gallibacterium anatis PCR positive samples were fixed in the formalin and dehydrated with gradient alcohol.The paraffin sections were made and stained with haematoxyylin and eosin,then were observed by lig...