Response surface optimization of medium components for producing cellulase by Cladosporium cladosporioides F4-1
枝状枝孢菌(Cladosporium cladosporioides)发酵产酶培养基的响应面法优化

The medium of liquid fermentation for producing CMCase (carboxymethyl cellulase) by Cladosporium cladosporioides strain F4-1 was optimized using response surface methodology (RSM). The optimum carbon, nitrogen and inorganic salts were obtained with one-factor-at-a-time. A central composite design and RSM were applied to determine the optimal concentration of each component above. The results show that the optimal medium was as follows:wheat straw 23.18 g·L～(-1),wheat bran 30.00 g·L～(-1), yeast extract 13.62 g·L～(-1),KH_2PO_4 4.60 g·L～(-1),NaCl 4.60 g·L～(-1),MgSO_4·7H_2O 0.46 g·L～(-1) . Under this condition, the CMCase activity reached 2.80 U·mL～(-1), which increased 2.18 times than before optimization.