Isolation and identification of a decabromodiphenyl ether degrading bacterium BD-24 and its degradation characteristics
十溴二苯醚降解菌的分离鉴定及其降解特性的初步研究

A bacterial strain BD-24,which was able to use decabromodiphenyl ether (BDE-209) as the sole carbon and energy source,was isolated from activated sludge in an oil refinery at Hangzhou,China by selective enrichment method .Based on morphological,physiological and biochemical characteristics,phylogenetic analysis of 16S rDNA gene sequence similarity,and (G + C) mol%,strain BD-24 was identified as Bacillussp . High performance liquid chromatograph analysis showed that the residue rate of BDE-209 (20 mg.L-1 ) in mineral medium decreased to75.1 % and65.6 % after the incubation of strain BD-24 for15 and25 days,respectively . Addition of second carbon sources generally enhanced the BDE-209 degradation by strain BD-24,especially in which maltose could result in 20 mg.L-1 of BDE-209 decreasing quickly to83.5 % within5 days . No active effect of different kinds of nitrogen sources on the BDE-209 biodegradation was observed .