Pure and Mixed Enantiomer of Moxifloxacin Hydrochloride Studied by NMR Spectroscopy
盐酸莫西沙星对映体及混合体的核磁共振研究

The structures of pure and mixed enantiomer of moxifloxacin hydrochloride were elucidated by NMR.The chemical shift of the spectral peaks were assigned using 1H-and 13C NMR,COSY,HSQC,HMBC and 2D NOESY techniques,as well as 19F-13C coupled splitting.It was found that 19F-13C coupling exists not only in the six-membered ring,but also in the five-membered heterocyclic ring,and that 4JFC in the five-membered heterocyclic ring is larger than 3JFC and 4JFC in the six-membered ring.The spectral characteristics of the pure and mixed enantiomer of moxifloxacin hydrochloride were summarized.A method was proposed to differentiate the pure and mixed enantiomer of moxifloxacin hydrochloride.