Identification of proteins interacting with hCLP46 from Human leukocyte cDNA Library using yeast two-hybrid system
酵母双杂交技术筛选与hCLP46蛋白相互作用的蛋白

Objective To screen the interactive proteins of hCLP46 and provide clues for the studies on hCLP46 function and pathogenesis of MDS-AML. Methods The human leukocyte cDNA library was screened with with pGBKT7-hCLP46 as bait plasmid by yeast two hybrid system , and Leu+, Mel+, Trp+, His+ positive yeast clones were obtained , The inserted fragments of identified positive clones were sequenced and analyzed with Blast in NCBI. Results There are 86 positive yeast clones which are obtained through screening the human leukocyte cDNA library with pGBKT7-hCLP46 as bait plasmid. Four different candidate gene sequences are obtained by Blast analysis in NCBI. Conclusion Four gene sequences were obtained , these coding proteins can interact with hCLP46 ,which may be involved with pathogenesis of MDS-AML.