Cloning and Bioinformatics Analysis of PmNHX1 gene fromXinjiang Halophyte Plantago maritima
新疆盐生植物车前PmNHX1基因的克隆及生物信息学分析

The salt damage to the plants is mainly caused by Na+. The catalytic transport of Na+/H+antiporter protein causes Na+ to come out of the Na+ compartmentalization of the vacuole membrane and the plasma membrane of the cell. The halophyte plants have been found to be resistant to salt stress caused by Na+. The conservative transport protein gene sequence of halophyte, Plantago maritima was carried out first time at Xinjiang by using RT-PCR and RACE technology and cloned into the Na+/H+ antiporter transport protein gene (2464 bp full-length cDNA) named as PmNHX1 (GenBank accession number: EU233808).It was found that the head of the gene consist the coding of the 1662 bp which encoded 553 amino acids. It had the molecular weight of 61.16 KDa and isoelectric point was 7.22. The data analysis showed that the protein mainly located in vacuole membrane was from 12 conservative sequence of the transmembrane domain of which the TM3 transmembrane domain (LFFIYLLPPI - putative amiloride binding domain) was found to be responsible for playing a competitive role. The amino acid homology of PmNHX1 and other plants antiporter protein was 64% - 80%. Due to its unique ability to find out physical and chemical properties and forecast the function of the gene, the use of bioinformatics methods laid the foundation for study related to identification of the salt-tolerant gene function of PmNHX1.