Cell-based Screening of Phage Antibody Library
基于细胞的噬菌体抗体库筛选技术

The limited number and low immunogenicity of tumor antigen make it difficult to screen out valuable phage antibody by panning on purified immobilized antigen because of the alternation of protein conformation. Most of these antigens have complex structure and the transporting and localization are depended on the cell types and microenvironment. Cell panning is applicable to complex antigen in its natural state without an additional purification procedure, or the exact antigen is not even known. So it is widely used in selection of tumor specific antibody, especially internalizing antibody and antibody specific targeting to vascular endothelial antigen. The major problem encountered in cell panning is related to the high background binding of non-specific antibody. Many efforts had been made to improve the specificity and sensitivity of cell panning. The combination of high throughout flow cytometry, combinatorial chemistry and proteome research will make this strategy more practical and fruitful. Here we outlined the progress of panning on intact cells and tissue sections to provide beneficial reference for understand and design of cell based panning.